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Home Новости Клиническая оценка Bruker Microflex MS против bioMérieux VITEK MS, два MALDI-TOF метода, для идентификации клинически значимых патогенных микроорганизмов.

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Клиническая оценка Bruker Microflex MS против bioMérieux VITEK MS, два MALDI-TOF метода, для идентификации клинически значимых патогенных микроорганизмов.

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Clinical evaluation of Bruker Microflex MS versus bioMerieux VITEK MS, two matrix-assisted laser

desorption ionization-time of flight mass spectrometry (MALDI-TOF) methods, for the identification of

clinically significant pathogens.

 

 

 

Клиническая оценка Bruker Microflex MS против bioMérieux VITEK MS, два MALDI-TOF метода, для идентификации

клинически значимых патогенных микроорганизмов.


 

 

Вывод: Обе системы отлично обеспечивают точный диагноз на 24-48 часов

быстрее, чем стандартные методы. Оба они могут быть легко включены в рутинные диагностические процедуры, потому что

удобны ( VITEK MS более чем Bruker Microflex MS), имеют короткое время выдачи результата и низкие затраты на анализ.

 

 

Vincent O. Rotimi1,2*, Rola Saleem2, Wafaa Jamal1,2, Aleyamma Pazhoor2, ZU Khan1,2, M. John Albert1,2

Departments of Microbiology, 1Faculty of Medicine, Kuwait University and 2Mubarak Al Kabir Hospital,

Kuwait

Objective: To evaluate two commercially available MALDI-TOF MS systems, Microflex MS® (MMS;

Bruker) and VITEK MS® (VMS; bioMerieux) for identification (ID) of clinically significant pathogens.

Methods: Clinically relevant broad diversity of bacterial and fungal isolates obtained during a 5-

consecutive month of routine laboratory processing of clinical specimens were subjected to ID by the

MMS and VMS in parallel with conventional phenotypic system (CPS; VITEK 2). For the MMS the

isolates were tested in duplicates directly and after pretreatment; yeasts were pretreated with 25% formic

acid. Identification was provided with accompanying scores (<1.7=no reliable ID; ≥1.7-<2.0=ID at genus

level; ≥2.0=ID at species level). For ID with VMS single deposits of the same sets of isolates were tested

in duplicates directly on MALDI-plate. Species were separated by a threshold of 50% matching peaks.

Interpretation of results was according to the manufacturers’ protocols. Discrepant results were resolved

one way or the other by 16S rRNA gene sequencing.

Results: A total of 1007 pathogens comprising 503 Gram-negative bacilli (GNB), 16 Gram-negative cocci

(GNC), 266 Gram-positive cocci (GPC), 20 Gram-positive bacilli (GPB), 188 yeasts and 9 molds, made

up of 48 genera and 102 species were tested. MMS and VMS correctly ID 98.1% and 99.3%, and 92.8%

and 96.3% of the isolates to genus and species levels, respectively. Both systems as well as the CPS

correctly ID all species of the family Enterobacteriaceae, Pseudomonas spp., and Acinetobacter

baumannii. And all Gram-positives to genus level. All Candida albicans and other species were correctly

ID by both systems. Discrepant results were resolved by 16S rRNA sequencing more in favor of VMS.

MMS miss-identified 2 S. mitis and S. pneumoniae. One strain identified as Ralstonia picketti by VITEK

2 and VMS was identified as R. insidiosa and R. solanaceavum by sequencing.

Conclusion: Both systems performed excellently well in terms of providing accurate diagnosis 24-48h

earlier than the CPS. Both can be easily incorporated into routine diagnostic procedures because they are

user-friendly (VMS>MMS), have short turn-around time and the cost analysis is low.

 

Обновлено для 13.08.2012 16:35